In the Seinfeld episode "The Opera", as Jerry and Elaine are waiting outside the opera house, Jerry starts singing "This is it" to pass the time to which Elaine laments to him by saying, "You know, it is so sad that all of your knowledge of high culture comes from Bugs Bunny cartoons."

When Warner Bros. released their video series "Golden Jubilee" in 1985, featuring the classic cartoons, the opening sequence shows Taz maniacally riding a motorcycle down a city street chased by a police car.

Beginning in January 2021, the original "This Is It" opening sequence was included in Bugs Bunny and Friends, part of MeTV's Saturday Morning Cartoons block.